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Microtiter plate methods

Enumeration of PAH-degraders in soil and detection of microbial growth on polycyclic aromatic hydrocarbons in microtiter plates using the respiration indicator WST-1.

Microplate test for growth on PAHs (pdf)
Protocols for test of growth on PAHs, MPN-enumeration of PAH-degraders and statistical evaluation of MPN-results. New version June 14 2006
MPN Calculator 1. (exe)

DOS-based program, downloadable, for calculation of MPN 

MPN Calculator 2.
Link to a website offering evaluation of data from MPN-enumeration.
Bacteriological Analytical Manual On-line.
Link to a website, offering advice on how to eveluate data from MPN-enumeration, including how to handle improbable results (e.g.,4-4-5-2-1-0-0-0).
AEM Paper (pdf)
Free down-load of the original article in Applied and Environmental Microbiology describing and validating the PAH-microplate method.
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Hans Brúsch

01.11.2007


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